Nuclear behavior in exconjugants of Paramecium bursaria
Abstract
Paramecium bursaria is the representative of “bursaria” group of Paramecium, whose nuclear behaviour during conjugation is quite different from other species. In its exconjugants, there are three types of nuclei, a parental macromicleus (pMac), two macronuclear anlagen (MA) and two presumptive micronuclei (Mic). According to the previous studies, both Mic and MA distribute equally during exconjugant division — each daughter cell receiving a Mic and a MA, which never happens in other Paramecium species studied so far. Meanwhile pMac sustains even in daughter cells. To clarify these issues, nuclear behavior in exconjugants of P. bursaria was studied by staining with carbol fuchsin solution. As the results, degeneration of one presumptive Mic as well as pMac in P. bursaria exconjugant was observed indicating different nuclear development pattern from previous reports.
Downloads
References
2. Gao X, Zhang X, Yang X. Morphological apoptotic characteristics of the post-meiotic micronuclei in Paramecium caudatum. Europ J Protistol 2010, 46: 243-250.
3. Gao X, Shi X, Yang X. Dynamic behaviour of stationary pronuclei during their positioning in Paramecium caudatum. Europ J Protistol 2011, 47(3): 235-237.
4. Gao X, Yang X, Zhu J, Yuan J, Wang Y, Song M. New details indicated by different stainings during conjugation of ciliated protozoa Paramecium. Zool Res. 2011, 32: 651-656.
5. Gao X, Zhu J, Yang X, Yuan J, Wang Y, Song M. Localization of stationary pronuclei during the congjugation of Paramecium indicated by immunofluorescence staining. Zool Res. 2011, 32: 461-464.
6. Wang Y, Yuan J, Gao X, Yang X. Stage-specific appearance of cytoplasmic microtubules around the surviving nuclei during the third prezygotic division of Paramecium. Zool Res 2012, 33(E5-6): E97-E102.
7. Yang X, Shi X. Gametic nuclear exchange during the conjugation of Paramecium polycaryum. JPN J Protozool. 2007, 40: 113-121.
8. Yang X, Takahashi M. Stage-related profiles of the reaction of the cells to heat shock during meiotic prophase in Parameicum caudatum. J Exp Zool 1996, 275: 53-60.
9. Yang X, Takahashi M. Disturbance of the determination of germinal and somatic nuclei by heat shock in Paramecium caudatum. J Eukaryot Microbiol 1999, 46: 49-55.
10. Yang X, Takahashi M. Nutrient supply induces germinal nuclear selection in exconjugants of Paramecium caudatum. Proc Japan Acad 2000, 76: Ser B, 87-91.
11. Yang X, Takahashi M. Nuclei may anchor at specific locations during nuclear determination in Paramecium caudatum. Europ J Protistol 2002, 38: 147-153.
12. Yang X, Gao X, Shi X. Detection of haploid nuclear death in living Paramecium caudatum. JPN J Protozool 2007, 40: 123-130.
13. Watanebe T, Shi X, Liu G, Jin M. Cytolocical studies of conjugation and nuclear processes in Paramecium duboscqui Chatton & Brachon 1933. Europ J Protistol 1996, Suppl 1: 175-182.
14. Wichterman R. Conjugation and fate of exconjugants of zoochlorellae-free Paramecium bursaria. Anat Rec 1943, 87: 50.
15. Wichterman R. Recent discoveries of nuclear processes an sexual phenomema in paramecium. Turtox News 1944, 22: 105-110.
16. Wichterman R. Direct observation of the transfer of pronuclei in living conjugants of Paramecium bursaria. Science 1946, 104: 505-506.
17. Wichterman R. The time schedule of mating and nuclear events in the conjugation of Paramecium bursaria. Turtox News 1948, 26: 2-10.
18. Sun X, Wu J, Gao X, Yang X. To enhance the production of magnetic iron-dextran particles by ultrasonic treatment and its applications in ciliate studies. Chn J Zool, 2010, 45(1): 90-93. (in Chinese)
19. Lin Y, Gao X, Yang X. A method for observation of living Paramecium. Shichuan J Zool 2009, 28: 276. (in Chinese)
20. Carr DH, Walker JE. Carbol fuchsin as a stain for human chromosomes. Stain Technol 1961, 36: 233-9236
21. Blackburn EH. Structure and function of telomeres. Nature 1991, 350:569-573.
22. Cech TR. The chemistry of self-splicing RNA and RNA enzymes. Science 236, 1532-1539.
23. Nanney DL. Nucleo-cytoplasmic interaction during conjugation in Tetrahymena. Biol Bull 1953, 105: 133-148.
24. Qiao J, Xu J, Bo T, Wang W. Micronucleus-specific histone H1 is required for micronuclear chromosome integrity in Tetrahymena thermophila. PLoS ONE 12(11): e0187475.
Copyright (c) 2018 IJRDO - Journal of Biological Science (ISSN: 2455-7676)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Author(s) and co-author(s) jointly and severally represent and warrant that the Article is original with the author(s) and does not infringe any copyright or violate any other right of any third parties, and that the Article has not been published elsewhere. Author(s) agree to the terms that the IJRDO Journal will have the full right to remove the published article on any misconduct found in the published article.
