The effects of PCR kits indicated during gene amplification of Panthera tigris amoyensis

  • Xianfu Zhang College of Animal Science and Technology, Zhejiang A&F University, 666 Wusu Street, Hangzhou 311300, China
  • Lin Zhao College of Animal Science and Technology, Zhejiang A&F University, 666 Wusu Street, Hangzhou 311300, China
  • Jianxun Liu Hangzhou Safari Park, 1 Jiulong Avenue, Hangzhou 311400, China
  • Weibin Shi Hangzhou Safari Park, 1 Jiulong Avenue, Hangzhou 311400, China
  • Keda Hua Bureau of Agriculture and Forestry, 118 Guihau West-Road, Hangzhou 311400, China
  • Yu Wang Hangzhou Safari Park, 1 Jiulong Avenue, Hangzhou 311400, China
  • Xianyu Yang College of Animal Science and Technology, Zhejiang A&F University, 666 Wusu Street, Hangzhou 311300, China
DOI: https://doi.org/10.53555/ar.v4i10.2540
Keywords: Panthera tigris Amoyensis, PCR, PCR kit, Thermal Cyclers

Abstract

It is not rare that no product could be detected after polymerase chain reaction (PCR). During the cloning of some DNA segments of South China tiger (Panthera tigris amoyensis), it was found that different PCR kits gave different amplification results. To clarify this issue, a series of PCR was carried out by adopting different PCR kits, PCR tubes and Thermal Cyclers from different manufactures. Among 4 DNA segments (A-D), D was amplified well regardless of PCR kits, A was amplified well only with real-time quantitative fluorescent PCR kit, meanwhile the amplification of B and C showed different levels depending on the PCR kits. All these results indicated the importance of PCR kits, while neither Thermal Cyclers nor PCR tubes affect the PCR products.

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Published
2018-10-30
How to Cite
Zhang, X., Zhao, L., Liu, J., Shi, W., Hua, K., Wang, Y., & Yang, X. (2018). The effects of PCR kits indicated during gene amplification of Panthera tigris amoyensis. IJRDO-Journal of Agriculture and Research (ISSN: 2455-7668), 4(10), 30-34. https://doi.org/10.53555/ar.v4i10.2540
Issue
Vol. 4 No. 10 (2018): IJRDO - Journal of Agriculture and Research | ISSN: 2455-7668
Section
Articles

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