Effect of Centrifugation on Competent Cells of Escherichia coli

  • Lin Zhao College of Animal Science and Technology, Zhejiang A&F University, 666 Wusu Street, Hangzhou, Zhejiang 311300, China
  • Hanxiao Zhao College of Animal Science and Technology, Zhejiang A&F University, 666 Wusu Street, Hangzhou, Zhejiang 311300, China
  • Hongxing Liu College of Animal Science and Technology, Zhejiang A&F University, 666 Wusu Street, Hangzhou, Zhejiang 311300, China
  • Xianyu Yang College of Animal Science and Technology, Zhejiang A&F University, 666 Wusu Street, Hangzhou, Zhejiang 311300, China
DOI: https://doi.org/10.53555/bs.v4i9.2426
Keywords: Escherichia coli, competent cells, Electrotransformation

Abstract

In this study, we changed centrifugation parameters (3 300 g, 2 min, 4°C) for cell harvesting and suspended cells with Pasteur pipettes during the preparation of E. coli competent cells for electrotransformation. As the result, the titer of either BL21 (DE3) or Top10 reached 109 easily, meanwhile at least 1 hr was saved for the whole process.

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References

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Published
2018-09-30
How to Cite
Zhao, L., Zhao, H., Liu, H., & Yang, X. (2018). Effect of Centrifugation on Competent Cells of Escherichia coli. IJRDO - JOURNAL OF BIOLOGICAL SCIENCE, 4(9), 18-20. https://doi.org/10.53555/bs.v4i9.2426
Issue
Vol. 4 No. 9 (2018): IJRDO - Journal of Biological Science
Section
Articles

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