METABOLIC ENGINEERING OF MICROORGANISM FOR THE PRODUCTION OF CARBOXYLIC ACID AND THEIR INHIBITORY EFFECTS.

  • HIRA SARFRAZ GC University Lahore, Pakistan
  • SIKANDER ALI GC University Lahore, Pakistan
  • JAVERIA MUSHTAQ GC University Lahore, Pakistan
  • FARZANA NAZIR GC University Lahore, Pakistan
Keywords: fermentation,, metabolic flux,, membrane properties,, metabolic engineering,, precursors, inhibitors.

Abstract

To fulfill the requirement of futurre coming perspective for chemicals like carboxylic acid that
are produced by microbial fermentation are an excellent biorenewable chemical. Due to their
demand and usage as precursor for a number of industrial chemical, it can be produced through
engineered microbes such as Saccharomyces cerevisiae, E.coli and Bascilus subtilis. Genetic
engineering is now successfully and commonly used to change carbon to ethanol the end
product of Saccharomyces cerevisiae to pyruvate. Genetic manipulation methods are useful for
pathway design and for the production of several organic acids. Deletions of metabolic
pathways that compete with the target compound are produces, can be a useful method for
redirecting metabolic flux into the desired pathways. However carboxylic acids become
increasingly inhibiting to the microorganisms. These inhibitors at their specific potency are
commonly used as food preservative. The effects of carboxylic acids include cell membrane
damage, change in microbial internal pH. Certain changes in cell membrane properties such as
fluidity, composition, integrity and intracellular pH and hydrophobicity are associated with
increase tolerance. These desirable products can use in the engineering of robust strains with
improved industrial performance.

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Author Biographies

HIRA SARFRAZ, GC University Lahore, Pakistan

Institute of Industrial Biotechnology,

SIKANDER ALI, GC University Lahore, Pakistan

Institute of Industrial Biotechnology,

JAVERIA MUSHTAQ, GC University Lahore, Pakistan

Institute of Industrial Biotechnolgy

FARZANA NAZIR, GC University Lahore, Pakistan

Institute of Industrial Biotechnolgy

Published
2017-06-30
How to Cite
SARFRAZ, H., ALI, S., MUSHTAQ, J., & NAZIR, F. (2017). METABOLIC ENGINEERING OF MICROORGANISM FOR THE PRODUCTION OF CARBOXYLIC ACID AND THEIR INHIBITORY EFFECTS. IJRDO -JOURNAL OF HEALTH SCIENCES AND NURSING, 2(6), 63-85. https://doi.org/10.53555/hsn.v2i6.1042