Vol 2 No 10 (2016): IJRDO - Journal of Biological Science
Articles

Molecular Detection and Diagnosis of Phytoplasma Infection in Cassava (Manihot esculenta Crantz) in Eastern Visayas, Philippines

Vasquez, Erlinda A
PhilRootcrops, Visayas State University,
Borines, Lucia M
Visayas State University
Bio
Palermo, Lady Fatima G
Visayas State University
Bio
Published October 31, 2016
Keywords
  • cassava,,
  • phytoplasma,
  • DNA extraction,
  • PCR detection,
  • Philippines
How to Cite
Erlinda A, V., Lucia M, B., & Fatima G, P. L. (2016). Molecular Detection and Diagnosis of Phytoplasma Infection in Cassava (Manihot esculenta Crantz) in Eastern Visayas, Philippines. IJRDO - Journal of Biological Science (ISSN: 2455-7676), 2(10), 30-48. Retrieved from https://ijrdo.org/index.php/bs/article/view/1669

Abstract

Phytoplasma disease is a threat to the cassava industry but little is known about
the disease in the country. This study was conducted to develop and optimize a protocol
for the detection of phytoplasma and determine the existence of phytoplasma strains in
the different cassava growing areas in Eastern Visayas, Philippines.
PCR reaction using primer pair PA2F/R and nested primer pair NPA2F/R as
well as the use of primer pair P1/P7 followed by nested primer pair R16F2n/R2 with
slight modifications in the thermocycling conditions detected phytoplasma infecting
cassava.
Ten out of the eleven areas in Eastern Visayas, a scale insect and a cassava
grown from tissue culture tested positive for phytoplasma using nested primer pairs
NPA2F/R and R16F2n/R16R2. Six band patterns were detected when NPA2F/R primer
amplicons were digested with RsaI and MspI restriction enzymes suggesting that more
than one strain of phytoplasma infect cassava.

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