Molecular Detection and Diagnosis of Phytoplasma Infection in Cassava (Manihot esculenta Crantz) in Eastern Visayas, Philippines

  • Vasquez, Erlinda A PhilRootcrops, Visayas State University,
  • Borines, Lucia M Visayas State University
  • Palermo, Lady Fatima G Visayas State University
DOI: https://doi.org/10.53555/bs.v2i10.1669
Keywords: cassava,, phytoplasma, DNA extraction, PCR detection, Philippines

Abstract

Phytoplasma disease is a threat to the cassava industry but little is known about
the disease in the country. This study was conducted to develop and optimize a protocol
for the detection of phytoplasma and determine the existence of phytoplasma strains in
the different cassava growing areas in Eastern Visayas, Philippines.
PCR reaction using primer pair PA2F/R and nested primer pair NPA2F/R as
well as the use of primer pair P1/P7 followed by nested primer pair R16F2n/R2 with
slight modifications in the thermocycling conditions detected phytoplasma infecting
cassava.
Ten out of the eleven areas in Eastern Visayas, a scale insect and a cassava
grown from tissue culture tested positive for phytoplasma using nested primer pairs
NPA2F/R and R16F2n/R16R2. Six band patterns were detected when NPA2F/R primer
amplicons were digested with RsaI and MspI restriction enzymes suggesting that more
than one strain of phytoplasma infect cassava.

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Author Biographies

Borines, Lucia M, Visayas State University

Department of Pest Management

Palermo, Lady Fatima G, Visayas State University

PhilRootcrops,

Published
2016-10-31
How to Cite
Erlinda A, V., Lucia M, B., & Fatima G, P. L. (2016). Molecular Detection and Diagnosis of Phytoplasma Infection in Cassava (Manihot esculenta Crantz) in Eastern Visayas, Philippines. IJRDO - JOURNAL OF BIOLOGICAL SCIENCE, 2(10), 30-48. https://doi.org/10.53555/bs.v2i10.1669
Issue
Vol. 2 No. 10 (2016): IJRDO - Journal of Biological Science
Section
Articles

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