@article{Erlinda A_Lucia M_Fatima G_2016, title={Molecular Detection and Diagnosis of Phytoplasma Infection in Cassava (Manihot esculenta Crantz) in Eastern Visayas, Philippines}, volume={2}, url={https://ijrdo.org/index.php/bs/article/view/1669}, DOI={10.53555/bs.v2i10.1669}, abstractNote={<p>Phytoplasma disease is a threat to the cassava industry but little is known about<br>the disease in the country. This study was conducted to develop and optimize a protocol<br>for the detection of phytoplasma and determine the existence of phytoplasma strains in<br>the different cassava growing areas in Eastern Visayas, Philippines.<br>PCR reaction using primer pair PA2F/R and nested primer pair NPA2F/R as<br>well as the use of primer pair P1/P7 followed by nested primer pair R16F2n/R2 with<br>slight modifications in the thermocycling conditions detected phytoplasma infecting<br>cassava.<br>Ten out of the eleven areas in Eastern Visayas, a scale insect and a cassava<br>grown from tissue culture tested positive for phytoplasma using nested primer pairs<br>NPA2F/R and R16F2n/R16R2. Six band patterns were detected when NPA2F/R primer<br>amplicons were digested with RsaI and MspI restriction enzymes suggesting that more<br>than one strain of phytoplasma infect cassava.</p&gt;}, number={10}, journal={IJRDO - JOURNAL OF BIOLOGICAL SCIENCE}, author={Erlinda A, Vasquez, and Lucia M, Borines, and Fatima G, Palermo, Lady}, year={2016}, month={Oct.}, pages={30-48} }