VALIDATION OF AQUEOUS ULTRASOUND ASSISTED EXTRACTION METHOD BY USING FRESH LEAVES OF MORINGA OLEIFERA WITH CONVENTIONAL EXTRACTION METHOD.
Aqueous extraction from fresh leaves of Moringa oleifera by Ultrasound Assisted Extraction (UAE) method was proposed in the present study. The proposed method was occupying most of the features of the green extraction method described in different literature. From successive five batches, the efficiency and efficacy of the proposed extraction method was compared with the conventional extraction method and found satisfactory performance. The average % yield (extraction efficiency) of the proposed method was observed 17.72± 1.67% statistically similar to the conventional methanol and ethanol extraction method. However, presence of tested phytochemicals were observed higher than the conventional method. Antimicrobial study (zone of inhibition or efficacy of the crude extracts) of the proposed UAE method was performed against Staphylococcus aureus (14.8 ± 2.20 mm), Streptococcus pyogenes (17.3 ± 3.20 mm), Escherichia coli (15.5 ± 2.55 mm) and Shigella boydii (19.4 ± 2.99 mm) and found statistically similar antimicrobial activities with the conventional methanol and ethanol extracts. Based on the experiment the Aqueous UAE method may be standardized as an effective green extraction procedure.
Aja PM, Nwachukwu N, Ibiam UA, Igwenyi IO, Offor CE and Orji UO. 2014. Chemical Constituents of Moringa oleifera Leaves and Seeds from Abakaliki, Nigeria. American Journal of Phytomedicine and Clinical Therapeutics, 2(3):310-321.
Ajayi AO and Fadeyi TE. 2015. Antimicrobial Activities and Phytochemical Analysis of Moringa oleifera Leaves on Staphylococus aureus and Streptococcus species. American Journal of Phytomedicine and Clinical Therapeutics, 3(10): 643-653.
Ayoola GA, Coker HAB, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC and Atangbayila TO. 2008. Phytochemical Screening and Antioxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria. Tropical Journal of Pharmaceutical Research, September,; 7 (3): 1019-1024.
Baker J, Liu JP, Robertson EJ and Efstratiadis A. 1993. Role of Insulin-like Growth Factors in Embryonic and Postnatal Growth. Cell, 75: 73-82.
Bashir H S, Mohammed AM, Magsoud AS, Shaoub AM. 2014. Isolation and Identification of Two Flavonoids from Moringa oleifera (Leguminosae) Leaves. Journal of Forest Products & Industries, 3(5), 211-215.
Bauer AW, Kirby WM, Sherris JC and Turck M. 1966. Antibiotic susceptibility testing by a standard single disk method. Am J Clin Pathol, 45(4): 493-496.
Biswas AK, Hoque TS and Abedin MA.2016. Effects of moringa leaf extract on growth and yield of maize. Progressive Agriculture, 27 (2): 136-143.
Castillo JAT, García SRS, Ávila GCGM, Flores ABL, González EIS, Arzola VEA, Acosta RIT, Sáenz EO, Hernández EO and Díez AG. 2013. Moringa oleifera: phytochemical detection, antioxidants, enzymes and antifungal properties. International Journal of Experimental Botany, 82: 193-202.
Cheenickal M and Mendez RM. 2017. Phytochemical screening and the antimicrobial activity of the leaves of Azadirachta indica, A. Juss. International Journal of Scientific & Engineering Research, 8(5): 721-724.
Chemat F, Vian MA and Cravotto G.2012. Green Extraction of Natural Products: Concept and Principles. International Journal of Molecular Sciences, 13: 8615-8627.
Dash SP, Dixit S and Sahoo S. 2017. Phytochemical and Biochemical Characterizations from Leaf Extracts from Azadirachta Indica: An Important Medicinal Plant. Biochem Anal Biochem, 6: 323.
Fahal EM, Rani AMB, Aklakur MD, Chanu TI and Saharan N. 2018. Qualitative and Quantitative Phytochemical Analysis of Moringa oleifera (Lam) Pods. International Journal of Current Microbiology and Applied Sciences,7(5): 657-665.
Francine U, Jeannette U and Pierre RJ. 2015. Assessment of antibacterial activity of Neem plant (Azadirachta indica) on Staphylococcus aureus and Escherichia coli. Journal of Medicinal Plants Studies, 3(4): 85-91.
Hazali NB, Ali MABM, Ibrahim MB and Masri M. 2015. Determination of phytochemicals and vitamin content of underutilized Baccaurea angulata fruit. Journal of Pharmacognosy and Phytochemistry, 4(4): 192-196
Hussain F and Hussain MM. 2012. Cytotoxic Effect of Crude Extracts of Acacia Nilotica. International Journal of Pharmaceutical Sciences and Research, 3(6): 1652-1655.
Ishtiaq F, Farooq R, Farooq A, Siddique M, Shah H, Hassan MU, Shaheen MA. 2009. Application of ultrasound in pharmaceutics world. Appl. Sci. J. 6: 886-893.
Latha CR, Kavitha S and Sathya S. 2015. Antimicrobial Efficacy of Azadirachta indica Leaf Extracts. International Journal of Science and Nature, 6(3): 432-440.
Mahdi HJ, Yousif EM, Khan NAK, Mahmud R, Murugaiyah VAL and Asmawi MZB. 2016. Optimizing Extraction Conditions of Moringa oleifera Lam leaf for percent yield, total phenolics content, total flavonoids content and total radical scavenging activity. International Journal of Advanced Research, 4(11): 682-695.
Malki ALA and Rabey HAE.2015. The Antidiabetic Effect of Low Doses of Moringa oleifera Lam. Seeds on Streptozotocin Induced Diabetes and Diabetic Nephropathy in Male Rats, BioMed Research International, Volume 2015, Article ID 381040, 13 pages
Maxson ED and Rooney LW. 1972. Evaluation of methods for Tannin analysis in Sorghum Grain.American association of cereal Cherrism, inc., Minnesota 56121. 11pages.
Mukhtar MD and Tukur A. 2000. Antibacterial Activity of Aqueous and Ethanolic Extracts of P. stratiotes. Journal of the Nigerian Society for Experimental Biology, 1: 51-59
Nweze NO, and Nwafor FI.2014. Phytochemical, proximate and mineral composition of leaf extracts of Moringaoleifera Lam from Nsukka, south-Eastern Nigeria. IOSR Journal of Pharmacy and Biological Sciences, 9(1): 99-103.
Ogundele VA and Fadeyi OE.2015. Isolation, Characterization and Derivatization of Some Bioactive Components in Moringa Oleifera Leaves. Nat Prod Chem Res, 3(5): 189
Okumu MO, Mbaria JM, Kanja LW, Gakuya DW, Kiama SG and Ochola FO. 2016. Phytochemical profile and antioxidant capacity of leaves of Moringa oleifera (Lam) extracted using different solvent systems. Journal of Pharmacognosy and Phytochemistry, 5(4): 302-308.
Onyagbodor OA and Aprioku JS.2017. Moringa oleifera leaf extract inhibits diabetogenic effect of alloxan in rats. IOSR Journal Of Pharmac, 7(10): 07-12.
Sadat AFMN, Mizan MRB, Sultana A, Rahman MM and Azad MAK. 2018. Comparative study of the Antimicrobial Activity of Methanol Extract and Ultrasound Assisted Water Extract of the Leaves of Azadirachta indica. Rajshahi University Journal of Environmental Science, 7: 40-47.
Servan ES, Ionescu M, Matinca D, Maier CS and Bojita MT. 2011. Screening of the Antibacterial and Antifungal Activity of Eight Volatile Essential oils. Farmacia, 59(3): 440-446.
Tende JA, Ezekiel I, Dikko AAU, and Goji ADT.2011. Effect of Ethanolic leaves extract of Moringa oleifera on blood glucose levels of streptozocin-induced diabetics and normoglycemic Wistar rats. British Journal of Pharmacology and Toxicology, 2(1): 1-4.
Terblanche U, Semakalu CC, Mtunzi F and Pillay M. 2017. Screening of Variables Influencing Extraction Yield of Cotyledon orbiculata: 23 Full Factorial Design. International Journal of Pharmacognosy and Phytochemical Research, 9(3): 303-312
Toma M, Vinatoru M, Paniwnyk L and Mason TJ. 2001. Investigation of the effects of ultrasound on vegetal tissues during solvent extraction. Ultrason Sonochem, 8: 137-142.
Trease GE and Evans WC. 1989. Pharmacology. 11th Edition. BailliereTindall Ltd., London, pp:60 – 75. 16. Maxson E.D. and Rooney L.W. (1972).Evaluation of methods for Tannin analysis in Sorghum Grain.American association of cereal Cherrism, inc., Minnesota 56121. 11pages.
Usman AG, Ammani HM and Bala SS.2018. Comparative Antioxidative and Cytotoxic Activity of Extracts of Moringa Oleifera and its Mistletoe. IOSR Journal of Applied Chemistry, 11(1): 13-18.
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