Panthera tigris amoyensis Genomic DNA Extraction with simplified method

  • Jinghua Ma Hangzhou Safari Park, Hangzhou 311400, China
  • Yukun Jia College of Animal Science and Technology, Zhejiang A&F University, Hangzhou 311300, China
  • Ying Lin College of Animal Science and Technology, Zhejiang A&F University, Hangzhou 311300, China
  • Xianfu Zhang
  • Xianyu Yang
DOI: https://doi.org/10.53555/as.v5i11.3367
Keywords: Genomic DNA extraction, Tigers, Sheep, Cattle

Abstract

Nowadays, eukaryotic genomic DNA is mainly extracted by its corresponding kits purchased from many companies. In this paper, a method for isolation of Panthera tigris amoyensis genomic DNA has been tried, which is really simple and quick.

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Author Biographies

Xianfu Zhang

College of Animal Science and Technology, Zhejiang A&F University, Hangzhou 311300,
China
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of
Zhejiang Province, Hangzhou 311300, China
Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet
Technology, Hangzhou 311300, China

Xianyu Yang

College of Animal Science and Technology, Zhejiang A&F University, Hangzhou 311300,
China
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of
Zhejiang Province, Hangzhou 311300, China
Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet
Technology, Hangzhou 311300, China

References

[1] Nakayama H, Nishikada T. 1995. Saibokougaku (ISBN4-87962-149-8), Shujunsha Co. Ltd. pp117-121. (In Japanese)
[2] Maniatis T, Fritsch E, Sambrook J. 1982. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY. pp458-459.
[3] Sambrook J, Fritsch E, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd Edition, Cold Spring Harbor Press, NY. ppB.16, E.3-4.
[4] Zhang X, Zhao L, Ma J, Liu J, Shi W, Hua K, Wang Y, Yang X. The effects of PCR kits indicated during gene amplification of Panthera tigris amoyensis. IJRDO-Journal of Agriculture and Research 2018, 4(10): 30-34.
Published
2019-11-30
How to Cite
Jinghua Ma, Yukun Jia, Ying Lin, Xianfu Zhang, & Xianyu Yang. (2019). Panthera tigris amoyensis Genomic DNA Extraction with simplified method. IJRDO-Journal of Applied Science, 5(11), 32-33. https://doi.org/10.53555/as.v5i11.3367
Issue
Vol. 5 No. 11 (2019): IJRDO - Journal of Applied Science (ISSN: 2455-6653)
Section
Articles

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