• Ajit kumar Research scholor



Plant materials have been used for medical purposes in human life. Cancer diseases severely effect the human populations in worldwide. There is a constant challenge to find out the new therapies and drugs for treating cancer. The new focus is on finding anti cancer drugs with less side effects compared to current treatments such as chemotherapy. Historical use of plant materials might contain anticancer activities. This study evaluated three plant extracts on cancer cells. The essential oils extracts of Hedyotis corymbosa, Inula racemosa and Holostemma adakodien were studied against cancer cells. All the three plant extracts have shown dose dependent cancer cell killing activity.



Download data is not yet available.


1. Yang, Z., Wenyuan, G., and Kefeng, L., (2008). Chinese herbal medicine in the treatment of lung cancer. Asian Journal of Traditional Medicine; 3(1): 1-11.
2. Greenwell, M., and Rahman, P. K. S. M., (2015). Medicinal plants: Their use in Anti-cancer treatment. International Journal Pharmaceutical Sciences and Research; 6(10): 4103-4112.
3. David, P. J., (2000). Phytochemistry and medicinal plants. Phytochemistry; 56(3): 237-243.
4. Amr, A., Nassar. A., Ahmed, M., and Khalid, M. A., (2009). Anticancer and antioxidant activity of some Egyptian medicinal plants. Journal of Medicinal plants Research; 3(10): 799-808.
5. Tamrat, T., and Yesudass, D. R., (2018). A Review on Anticancer activity of some plant-Derived compounds and their mode of Action. Natural Products Chemistry & Research; 6(4): 1-9.
6. Rita, C. S., Luciana, N. A., and Damiao, P. S., (2013). A Review on anti-inflammatory activity of monoterpenes. Molecules; 13: 1227-1254.
7. Lamson, D. W., and Brignall, M. S. (2000). Antioxidants and Cancer, part 3: Quercetin. Alternative Medicine Review; 5(3): 196-208.
8. Durcy, R. C., Luis C. G., Leovigildo, Q., and Enrique, S. D (2017). Chemical composition and antioxidant DPPH activity of the Floral and Leaves essential Oils of Montanosa speciosa DC. American Journal of Plant Sciences; 8(4): 745-753.
9. Olamide, E. A., Funsho, O. O., Tan, N. H., and Zeng, G., (2017). In vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia. Journal of Basic and Applied Sciences; 6(1): 10-14.
10. Djacbou, D. S., Pieme, C. A., Biapa, P. C., and Penlap, B. V., (2014). Comparison of in vitro antioxidant properties of extracts from three plants used for medicinal purpose in Cameroon: Acalypha racemosa, Garcina lucida and Hymenocardia lyrata. Asian Pacific Journal of Tropical Biomedicine; 4(2): 625-632.
11. Jiri, S., Tunde, J., Miroslav, P., Helena, S., Mojmir, B., Lenka, T., Stefan, B., Borivoj, K., Robert, P., Jiri, M., Zuzana, T., and Jan, S., (2014). Evaluation of Antioxidant activity, Polyphenolic Compounds, Amino acids and Mineral Elements of Representative Genotypes of Lonicera edulis. Molecules; 19: 6504-6523.
12. Surendra, K. S., and Ajay, P. S., (2012). In vitro antioxidant and free radical scavenging activity of Nardostachys jatamansi DC. Journal of Acupuncture and meridian studies; 5(3): 112-118.
13. McCord, J. M., and Fridovich, I. (1969). Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). The Journal of Biological Chemistry; 244(22): 6049-6055.
14. Wangchuk, P., Keller, P. A., Pyne, S. G., and Taweechotipatr, M., (2013). Inhibition of TNF-α production in LPS-activated THP-1 monocytic cells by the crude extracts of seven Bhutanese medicinal plants. Journal of Ethnopharmacology; 148 (3), 1013-1017.
15. Esteban, A. O., and Andrea, L. K., (2016). Sulforhodamine B (SRB) Assay in Cell Culture to investigate Cell Proliferation. Bioprotocol; 6 (21): 1-9.
16. Braga, P. A. C., Dos Santosa, D. A. P., Da Silva, M. F. D. G. F., Vieira, P. C., Fernandes, J. B., Houghton, P. J., and Fang, R., (2007). In vitro cytotoxicity activity on several cancer cell lines of acridone alkaloids and N-phenyletyl-benzamide derivatives from Swinglea glutinosa (Bl.) Merr. Natural Product Research: Formerly Natural Product Letters; 21(1): 47-55.
17. Durvanei, A. M., Manuela, G. L. D. S., Mario, C. C. J., and Itamar, R. G. R., (2014). Antiproliferative effect of the jararhagin toxin on B16F10 murine melanoma. BMC Complementary and Alternative Medicine; 14 (446): 1-13.
18. Mahmoud, H., Amin, D. F., Arezoo, Y., Reza, E. H., Kaveh T., Seyed, M. T., Sarvena, E. S., Konstantinos, T., Dimitrios, K., George, T., Dragana, N., Nikita, Y. A., Demetrios, A. S., Aristides, M. T., and Ramin, R., (2017). Anticancer and apoptosis inducing effects of quercetin in vitro and in vivo. Oncology Reports; 38: 819-828.
19. La-ongthong, V., and Suwanna, K., (2018). Cytotoxicity evaluation of a Thai herb using Tetrazolium (MTT) and Sulforhodamine B (SRB) assays. Journal of Analytical Science and Technology; 9(15): 1-6.
20. Kamran, G., Yosef, G., and Mohammad, A. E., (2009). Antioxidant activity, phenol and flavonoid contents of 13 citrus species peels and Tissues. Pakistan Journal of Pharmaceutical Sciences; 22(3): 277-281.
21. Jhon, M. C. G., (1985). Age pigments and free radicals: fluorescent lipid complexes formed by iron and copper containing proteins. Biochimica et Biophysica acta (BBA)- Lipid Metabolism; 834(2):144-148.
22. Jalal, P., Ahmad, S., and Enaytollah, S., (2016). Role of Oxygen Free radicals in Cancer Development and treatment. Intech Open access peer-reviewed chapter; Chapter 17: 1-16.
23. Seyfried, T. N., and Shelton, L. M., (2010). Cancer as a metabolic disease. Nutrition and metabolism; 7(7): 1-22.
24. Agarwal, B. B., Ichikawa, W., Garodia, P., Weeransindhe, P., Sethi, G., Bhatt, I. D., Pandey, M. K., Shishodia, S., and Nair, M. G., (2006). From traditional Ayurvedic medicine to modern medicine identification of therapeutic targets for suppression of inflammation and cancer. Expert opinion and Therapeutic Targets; 10(1): 87-118.
25. Sanchez-Moreno, C., (2002). Review: Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Science and Technology International; 8(3): 121-137.
26. Soobrattee, M. A., Neergheen, V. S., Luximon-Ramma, A., Aruoma, O. I., and Bahorun, T. (2005). Phenolics as potential antioxidant therapeutic agents: mechanism and actions. Mutation Research; 579 (1): 200-213.
27. Fridovich, I. (1978). The biology of oxygen radicals. Science Washington DC; 201:875–880.
28. Dimitrina, Z., Paraskev, N., and Gerassim, K., (2010). Radical scavenging and antioxidant activities of methanolic extracts from Hypericum species growing in Bulgaria. Pharmacognosy Magazine; 6(22): 74-78.
29. Schlesier, K., Harwat, M., Bohm, V., and Bitsch, R., (2002). Assessment of antioxidant activity by using different in vitro methods. Free Radical Research; 36(2): 177-187.
30. Mani, A. R., Pannala, A. S., Orie, N. N., Ollosson, R., Harry, D., Rice-Evans, C. A., and Moore, K. P. (2003). Nitration of endogenous para-hydroxyphe-nylacetic acid and the metabolism of nitrotyrosine. Biochem. Journal; 374(2): 521-527.
31. Kang, K. H., Kong, C. S., Seo, Y., Kim, M. M., and Kim, S. K. (2009). Anti-inflammatory effect of coumarins isolated from Corydalis heterocarpa in HT-29 human colon carcinoma cells. Food and Chemical Toxicology; 47(8): 2129-2134.
32. Dey, M., Ripoll, C., Pouleva, R., Dorn, R., Aranovich, I., Zaurov, D., Kurmukov, A., Eliseyeva, M., Belolipov, I., Akimaliev, A., Sodombekov, I., Akimaliev, D., Lila, M.A., and Baskin, I. (2008). Plant extracts from central asia showing anti-inflammatory activities in gene expression. Phytotherapy Research; 22(7): 929-934.
33. Rose, P., Won, Y. K., Ong, C. N., and Whiteman, M. (2005). β-Phenylethyl and 8- methylsulphinyloctyl isothiocyanates constituents of watercress suppress LPS-induced production of nitric oxide and prostaglandin E2 in RAW 2647 macrophages. Nitric Oxide; 12(4): 237-243.
34. Laupattarakasem, P., Wangsrimongkol, T., Surarit, R., and Hahnvajanawong, C. (2006). In vitro and in vivo anti-inflammatory potential of Cryptolepis buchanani. Journal of Ethnopharmacology; 108(3): 349-354.
35. Vichai, V., and Kirtikara, K., (2006). Sulforhodamine B colorimetric assay for screeing. Nature protocols; 1(3): 1112-1116.
36. Ali, M. A., Abul, F. M., Al-Hemaid, F. M., and Abou-Tarboush, F. M., (2013). In vitro cytotoxicity screening of wild plant extracts from Saudi Arabia on Human breast adenocarcinoma cells. Genetics and Molecular Research; 13(2): 3981-3990.
37. Yu, H. W., Wai, Y. T., Chin, P. T., Kamariah, L., and Kar, L. N., (2014). Cytotoxic activity of Kenaf (Hibiscus cannabinus L.) seed extract and oil against human cancer cell lines. Asian Pacific Journal of Tropical Biomedicine; 4(1): 510-515.
How to Cite
kumar, A. (2019). The INVITRO ANTICANCER ACTIVITY OF MEDICINAL PLANTS. IJRDO-Journal of Applied Science, 5(2), 36-46.