StASR 2 Overexpression Decreases Salicylic Acid Accumulation Leading to Lower Stomatal Conductance in Nicotiana tabacum

  • Wu Hanyu
Keywords: ASR, gene, stoma, salicylic acid, ATP


Plant ASRs (ABSCISIC ACID STRESS RIPENING ) contains conserved domains in response to ABA and water deficiency, which function on fruit development and drought response. Overexpression of ASR leads to stoma close, enhancing plant tolerance to drought. However, it is unknown yet that how ASR regulates stoma close or open. In this study, morphological and physiological phenotypes were assayed with transgenic lines overexpressing StASR 2 cDNA from potato in Nicotiana tabacum. Data analysis showed that overexpressing StASR 2 resulted in decrease on stoma conductance and transpiration ratio, attributing to a low salicylic acid accumulation level. Although accumulations of chlorophyll and ATP intensified in StASR 2 overexpression lines, net photosynthesis ratio was attenuated owing to reduced stoma conductance. Meanwhile, StASR 2 overexpression leaflets no long appeared sensitivity to ABA treatment and no significant changes on leaf ABA contents was detected between StASR 2 overexpression and control. The result indicates that less salicylic acid accumulation mediates involvement of StASR 2 overexpression in stomatal close rather than ABA.


Download data is not yet available.


Agnihotri A, Gupta P, Dwivedi A, Seth C.S. Counteractive mechanism (s)
of salicylic acid in response to lead toxicity in Brassica juncea (L.) Czern.
cv. Varuna. Planta. 2018, 21. doi: 10.1007/s00425-018-2867-0.
Dominguez P.G, Carrari F. ASR1 transcription factor and its role in
metabolism. Plant Signal Behavior. 2015, 10(4):e992751.
El-Esawi M.A, Elansary H.O, El-Shanhorey N.A, Abdel-Hamid A.M.E, Ali
HM, Elshikh M. Salicylic Acid-Regulated Antioxidant Mechanisms and
Gene Expression Enhance Rosemary Performance under Saline
Conditions. Front Physiology. 2017, 21;8:716. doi:
Golan I, Dominguez P.G, Konrad Z, Shkolnik-Inbar D, Carrari F, Bar-Zvi D.
Tomato ABSCISIC ACID STRESS RIPENING (ASR) gene family revisited.
PLoS One. 2014, 13;9(10):e107117.
Jia H, Jiu S, Zhang C, Wang C, Tariq P, Liu Z, Wang B, Cui L, Fang J.
Abscisic acid and sucrose regulate tomato and strawberry fruit ripening
through the abscisic acid-stress-ripening transcription factor. Plant
Biotechnol J. 2016, 14(10):2045-65.
Joo J, Lee Y.H, Kim Y.K, Nahm BH, Song SI. Abiotic stress responsive rice
ASR1 and ASR3 exhibit different tissue-dependent sugar and
hormone-sensitivities. Moleular Cells. 2013, 35(5):421-35.
Kim Y, Mun B.G, Khan A.L, Waqas M, Kim H.H, Shahzad R, Imran M, Yun
B.W, Lee I.J. Regulation of reactive oxygen and nitrogen species by
salicylic acid in rice plants under salinity stress conditions. PLoS One.
2018, 20;13(3):e0192650.
Li J, Li Y, Yin Z, Jiang J, Zhang M, Guo X, Ye Z, Zhao Y, Xiong H, Zhang Z,
Shao Y, Jiang C, Zhang H, An G, Paek NC, Ali J, Li Z. OsASR5 enhances
drought tolerance through a stomatal closure pathway associated with
ABA and H2O2 signalling in rice. Plant Biotechnology Journal. 2017,
Liu J.F, Feng X.J, Cui X.Q, Cai Q, Yao X.L. Expression of E. coli glgB in
Potatoes Generating Tuber Starches with Various Features. Molecular
Plant Breeding. 2012, 3:6, 57-62.10
Ma X, Zheng J, Zhang X, Hu Q, Qian R. Salicylic Acid Alleviates the
Adverse Effects of Salt Stress on Dianthus superbus (Caryophyllaceae) by
Activating Photosynthesis, Protecting Morphological Structure, and
Enhancing the Antioxidant System. Front Plant Sci. 2017, 21;8:600. doi:
Merilo E, Yarmolinsky D, Jalakas P, Parik H, Tulva I, Rasulov B, Kilk K,
Kollist H. Stomatal VPD Response: There Is More to the Story Than ABA.
Plant Physiol. 2018, 176(1):851-864.
Mimouni H, Wasti S, Manaa A, et al. Does Salicylic Acid (SA) Improve
Tolerance to Salt Stress in Plants? A Study of SA Effects On Tomato Plant
Growth, Water Dynamics, Photosynthesis, and Biochemical Parameters.
OMICS. 2016 , 20(3):180-90.
Miura K, Tada Y. Regulation of water, salinity, and cold stress responses
by salicylic acid. Front Plant Science. 2014, 23;5:4.
Okuma E, Nozawa R, Murata Y, Miura K. Accumulation of endogenous
salicylic acid confers drought tolerance to Arabidopsis. Plant Signal
Behavior. 2014, 9(3):e28085.
Sedaghat M, Tahmasebi-Sarvestani Z, Emam Y, Mokhtassi-Bidgoli A.
Physiological and antioxidant responses of winter wheat cultivars to
strigolactone and salicylic acid in drought. Plant Physiology Biochemistry.
2017, 119:59-69.
Tang Y, Sun X, Wen T, Liu M,Yang M, Chen X. Implications of terminal
oxidase function in regulation of salicylic acid on soybean seedling
photosynthetic performance under water stress. Plant Physiology
Biochemistry. 2017, 112:19-28.