Prevention of swelling of liver tumor in Sprague-Dawley rat fed with the Kinu-Mozuku, an edible Japanese brown alga grown at seashores in Noto-peninsula.
Keywords:
Kinu-Mozuku, brown algae, fucoidan, Sprague-Dawley (SD) rat, outbred, liver tumor, HCC, diethylnitrosamine (DEN)
Abstract
Aim: Swelling of the tumor in the liver is difficult to stop by ordinary medicine. Then, new
and safe drug preventing tumor-growth is required.
Method: Three Sprague-Dawley rats, injected chemical carcinogen of diethyl- nitrosamine for
12 weeks, are then fed the edible brown-alga Kinu-Mozuku, grown at seashores around Noto
peninsula, Japan, for 3 weeks.
Results: No liver tumor is found in three Kinu-Mozuku-fed sample rat. On the contrary, all
the three control-rats, fed without Mozuku, show very large tumor in their livers. This
observation is statistically significant (P = 0.05 by Mann-Whitney’s U test). Conclusion: Japanese edible Kinu-Mozuku (Nemacystis decipiens) is proven to be the strong
foodstuff/drug preventing enlargement of liver-tumor in the outbred rat. Thus, the edible
fucoidan is expected to be the powerful and safe drug/medicine preventing and healing the
human HCC and also the other intractable human cancers.
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References
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hydrophobic proteins in response to biotin administration in serum of infant patients with
alopecia as assessed by the protein micro-sequencing method.
Journal of Microbial & Biochemical Technology, 7, 39-46.
http://dx.doi.org/10.4172/1948-5948.1000179
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Effects of taking of edible fucoidan for long-time and for excess- amount to the rats.
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milks as assessed by high-performance affinity chromatography. Clinical Pediatric
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Hayakawa, K., & Nagamine, T. (2009). Effect of fucoidan on the biotinidase kinetics in human
hepatocellular carcinoma. Anticancer Research, 29, 1211-1218.
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in HepG2: effect of fucoidan is not due to gene expression. Cancer Genomics &
Proteomics, 11, 93-114.
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acids. Nobel Lecture, 11 December.
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Nagae, H. (1990) Effects of SSM, an extract from human type tubercle bacilli, on syngenic
guinea pig tumors. Nippon Ika Daigaku Zasshi, 57, 235-243 (in Japanese).Nagamine, T., Hayakawa, K., Nakazato, K., & Iha, M. (2015). Determination of the active
transport of fucoidan derived from Okinawa Mozuku across the human intestinal
Caco-2 cells as assessed by size-exclusion chromatography. Journal of
Chromatography B, 997, 187-193.
http://dx.doi.org/10.1016/j.jchromb.2015.05.026
Nilsson, L., & Kågedal, B. (1993). Co-purification of human serum lipoamidase and
biotinidase: evidence that the two enzyme activities are due to the same enzyme
protein. Biochemical Journal, 291, 545-551.
Oizumi, J., & Hayakawa, K. (1989). Liberation of lipoate by human serum lipoamidase from
bovine heart pyruvate dehydrogenase. Biochemical and Biophysical
Research Communications, 162, 658-663.
Schiffer, E., Housset, C., Cacheux, W., Wendum, D., Desbois-Mouthon, C., Rey, C., …
Rosmorduc, O. (2005). Gefitinib, an EGFR inhibitor, prevents hepatocellular
carcinoma development in the rat liver with cirrhosis. Hepatology, 41, 307-314.
Snedecor, G.W., & Cochran, W.G. (1967). Statistical Methods. 6th ed. Ames: The Iowa State
University Press.
Snell, E.E., Strong, F.M., & Peterson, W.H. (1937). Growth factors for bacteria. VI:
Fractionation and properties of an accessory factor for lactic acid bacteria.
Biochemical Journal, 31, 1789–1799.
Stanley Tsai, C., Burgett, M.W., & Reed, L.J. (1973). α-Keto acid dehydrogenase complexes
XX. A kinetic study of the pyruvate dehydrogenase complex from bovine
kidney. Journal of Biological Chemistry, 284, 8348-8352.
hydrophobic proteins in response to biotin administration in serum of infant patients with
alopecia as assessed by the protein micro-sequencing method.
Journal of Microbial & Biochemical Technology, 7, 39-46.
http://dx.doi.org/10.4172/1948-5948.1000179
Abe, N., Kawamoto, H., Miki, Y., Kimura, T., Kasagi, K., Ichikawa, O., … Ikeda, T. (2009).
Effects of taking of edible fucoidan for long-time and for excess- amount to the rats.
The Proceedings of the Joined Meeting in Okinawa by Japan Society for Bioscience,
Biotechnology, and Agrochemistry, Japan Society of Nutrition and Food Science, and
Japan Food Science and Engineering Society, p 16, 31 October (orally presented in
Japanese).
Berteau, O., & Mulloy, B. (2003). Sulfated fucans, fresh perspectives: structures, functions,
and biological properties of sulfated fucans and an overview of enzymes active
toward this class of polysaccharide. Glycobiology, 13, 29-40.
Chauhan, J., & Dakshinamurti, K. (1986). Purification and characterization of human serum
biotinidase. Journal of Biological Chemistry, 261, 4268-4275.
Cory, A.H., Owen, T.C., Barltrop, J.A., & Cory, J.G.. (1991). Use of an aqueous soluble
tetrazolium/formazan assay for cell growth assays in culture. Cancer
Communications, 3, 207-212.
Harris, K.M., Lenz, P., Hankey, K.G., MacVittie, T., Farese, A., Nakajima, K., … Mann, D.L.
(2008). Products of anti-CD3/anti-CD28 activated lymphocytes induce
differentiation and maturation of dendritic cells and have adjuvant-like activity in
vitro and in vivo. Clinical Immunology, 129, 58-68.Hayakawa, K., Kusaka, I., & Fukui, S. (1975). Resistance to mercuric chloride in Pseudomonas
K-62. Agricultural and Biological Chemistry, 39, 2171-2179.
Hayakawa, K., & Oizumi, J. (1988 a). Human serum lipoamidase. Enzyme, 40, 30-36.
Hayakawa, K., & Oizumi, J. (1988 b). Human serum biotinidase is a thiol-type enzyme.
Journal of Biochemistry, 103, 773-777.
Hayakawa, K., Yoshikawa, K., & Watanabe, T. (1994). Biotinidase: determinations of enzyme
activity, chemical structures such as glycochain structure and aminoacid sequence, and potential physiological roles of the enzyme and the possibility
of biotinidase onto clinical applications. Vitamins (Kyoto), 68, 318-320 (in
Japanese).
Hayakawa, K., Hirano, M., Oizumi, J., & Hosoya, M. (1998). Isoelectric focusing of
biotinidase and lipoamidase with the addition of non-ionic detergent. Analytica
Chimica Acta, 372, 281-289.
Hayakawa, K., Yoshinaga, T., Hirano, M., Yoshikawa, K., Katsumata, N., Tanaka, T., &
Nagamine, T. (2001). Protein determination by high-performance gel- permeation
chromatography: Applications to human pancreatic juice, human bile and tissue
homogenate. Journal of Chromatography B, 754, 65-76.
Hayakawa, K., Nagamine, T., Li, X-K., Katsumata, N., Ogata, T., & Tanaka, T. (2005).
Affinity chromatographic determination of D-aspartic acid in the liver cell lines.
Trends in Chromatography, 1, 105-110.
Hayakawa, K., Katsumata, N., Yoshikawa, K., Hirano, M., Ogata, T., Tanaka, T., & Nagamine,
T. (2007). Determination of lipoic (thioctic) acid by high-per-formance affinity chromatography with a trypsin-treated avidin-bound
column. Trends in Chromatography, 3, 31-42.
Hayakawa, K., Katsumata, N., Abe, K., Hirano, M., Yoshikawa, K., Ogata, T., …
Nagamine, T. (2009). Wide range of biotin (vitamin H) content in foodstuffs and powdered
milks as assessed by high-performance affinity chromatography. Clinical Pediatric
Endocrinology, 18, 41-49.
Hayakawa, K., & Nagamine, T. (2009). Effect of fucoidan on the biotinidase kinetics in human
hepatocellular carcinoma. Anticancer Research, 29, 1211-1218.
Hayakawa, K., & Nagamine, T. (2014). Fucoidan-dependent increased membrane component
in HepG2: effect of fucoidan is not due to gene expression. Cancer Genomics &
Proteomics, 11, 93-114.
Kögl, F., & Tönnis, B. (1936). Über das Bios-Problem. Darstellung von Krystallisierten Biotin
aus Eigelb. Hoppe-Seyler's Zeitschrift fur Physiologische Chemie, 242, 43–73 (in
German).
Kylin, H. (1915). Untersuchungen über die Biochemie der Meeresalgen. Hoppe-Seyler's
Zeitschrift fur Physiologische Chemie, 94, 337-425 (in German).
Lynen, F. (1964). The pathway from “activated acetic acid” to the terpenes and fatty
acids. Nobel Lecture, 11 December.
http://nobelprize.org/nobel_prizes/medicine/laureates/1964/lynen-lecture.pdf
Nagae, H. (1990) Effects of SSM, an extract from human type tubercle bacilli, on syngenic
guinea pig tumors. Nippon Ika Daigaku Zasshi, 57, 235-243 (in Japanese).Nagamine, T., Hayakawa, K., Nakazato, K., & Iha, M. (2015). Determination of the active
transport of fucoidan derived from Okinawa Mozuku across the human intestinal
Caco-2 cells as assessed by size-exclusion chromatography. Journal of
Chromatography B, 997, 187-193.
http://dx.doi.org/10.1016/j.jchromb.2015.05.026
Nilsson, L., & Kågedal, B. (1993). Co-purification of human serum lipoamidase and
biotinidase: evidence that the two enzyme activities are due to the same enzyme
protein. Biochemical Journal, 291, 545-551.
Oizumi, J., & Hayakawa, K. (1989). Liberation of lipoate by human serum lipoamidase from
bovine heart pyruvate dehydrogenase. Biochemical and Biophysical
Research Communications, 162, 658-663.
Schiffer, E., Housset, C., Cacheux, W., Wendum, D., Desbois-Mouthon, C., Rey, C., …
Rosmorduc, O. (2005). Gefitinib, an EGFR inhibitor, prevents hepatocellular
carcinoma development in the rat liver with cirrhosis. Hepatology, 41, 307-314.
Snedecor, G.W., & Cochran, W.G. (1967). Statistical Methods. 6th ed. Ames: The Iowa State
University Press.
Snell, E.E., Strong, F.M., & Peterson, W.H. (1937). Growth factors for bacteria. VI:
Fractionation and properties of an accessory factor for lactic acid bacteria.
Biochemical Journal, 31, 1789–1799.
Stanley Tsai, C., Burgett, M.W., & Reed, L.J. (1973). α-Keto acid dehydrogenase complexes
XX. A kinetic study of the pyruvate dehydrogenase complex from bovine
kidney. Journal of Biological Chemistry, 284, 8348-8352.
Published
2015-08-31
How to Cite
Hayakawa, K., & Nagamine, T. (2015). Prevention of swelling of liver tumor in Sprague-Dawley rat fed with the Kinu-Mozuku, an edible Japanese brown alga grown at seashores in Noto-peninsula. IJRDO - JOURNAL OF BIOLOGICAL SCIENCE, 1(4), 15-34. https://doi.org/10.53555/bs.v1i4.2359
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