Quantitative spectrophotometric estimation of specific monosaccharides by DNSA method

  • Karishma Rajbhar amniranjan Jhunjhunwala College,
  • Himanshu Dawda
  • Usha Mukundan
Keywords: Monosaccharides, Reducing sugars, Spectrophotometric, DNSA method.

Abstract

The determination of saccharide is widespread and has led to the development of numerous
analytical techniques for measuring its concentrations. Analytical tool with various applications
in different fields are tedious and have time-consuming protocols ranging from food analysis
to biology. The overall purpose of this research is to develop easy methods for analyzing
reducing sugars or saccarides. The most abundant monosaccharide in nature Dglucose
sometimes referred to as dextrose in our research its shows a peak difference of 1nm as one
water molecule is always loosely attached to dextrose ring structure. In this article it is seen
that DNSA methods has advantage of its simplicity, low cost, sensitivity and adoptable
during handling the analysis of a large number of samples at a time.

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Author Biographies

Karishma Rajbhar, amniranjan Jhunjhunwala College,

Karishma Rajbhar, Plant biotechnology laboratory, Ramniranjan Jhunjhunwala College,
Ghatkopar west, Mumbai 400 086, Maharashtra, India

Himanshu Dawda

Ghatkopar west, Mumbai 400 086, Maharashtra, India.

Usha Mukundan

This research was supported by a grant from Hindustan Unilever.

Published
2016-01-31
How to Cite
Rajbhar, K., Dawda, H., & Mukundan, U. (2016). Quantitative spectrophotometric estimation of specific monosaccharides by DNSA method. IJRDO - JOURNAL OF BIOLOGICAL SCIENCE, 2(1), 112-126. https://doi.org/10.53555/bs.v2i1.1592