• Irekhbayar Jambal Department of Chemistry, School of Arts and Sciences, National University of Mongolia (NUM), Baga toiruu, Sukhbaatar District, Ulaanbaatar 14201, Mongolia.
  • Amarjargal Ayurzana Department of Chemistry, Khovd branch school of National University of Mongolia, Jargalant soum,Khovd aimag, 84140, Mongolia
  • Altantsetseg Shatar Institute of Chemistry and Chemical Technology, MAS, 4th Building Enkhtaivan Avenue, Ulaanbaatar 210351, Ulaanbaatar, Mongolia
Keywords: A.frigida Willd, essential oil, antimicrobial, antioxidant, cytotoxic activities


Medicinal plants have always been considered a healthy source of life for all people. In Mongolia, the use of various medicinal and food plants has a long history. In recent years, interest in plant-derived food additives has grown. This study was designed to determine essential oil chemical composition and evaluate antioxidant, cytotoxic activities of aerial parts serial fractions from Artemisia frigida Willd. grown in Mongolia. The chemical composition of essential oil from A.frigida Willd. was determined by GC-MS analysis. Among them 40 components (35.07%), 1,8-cineol (27.22%), borneol (3.01%), terpin-4-ol (4.51%), γ-cadinene (7.5%), α-terpineol (2.98%), bornylacetate (1.80%) were found to be the major ones. The ethanol crude extract of A.frigida Willd. was suspended in water and consequently fractionated with n-hexane, chloroform, ethyl acetate and butyl alcohol. All the fractions were examined for their antioxidant and cytotoxic activities by using DPPH and MTT assays, respectively. Ethyl acetate fraction showed the highest DPPH radical scavenging activity at a concentration of 50 µg/ml by 93.17% with the IC50 value of 11.68 µg/ml. MTT results showed that water fraction demonstrated significant cytotoxicity against A549 - human alveolar basal epithelial cell line, with values of 78.69 and 60.98% (50; 10 µg/ml; at 48 h). These suggest that the fractions of A.frigida Willd. could hold a good potential for use in the pharmaceutical industry.


Download data is not yet available.


[1] Adams, R.P. (1991). Cedar Wood Oil - Analysis and properties. In: Modern Methods of Plant Analysis: Oils and Waxes. Springer-Verlag Berlin, Germany, 159-173.
[2] Azevedo, F.; Marques, F.; Fokt, H.; Oliveira, R.; Johansson, B. (2011). Measuring oxidative DNA damage and DNA repair using the yeast comet assay. Yeast, 28, 55-61.
[3] Adams, R.P. (2001). Identification of essential oils components by gas chromatography/mass spectroscopy. Carol Stream, Il, USA: Allured Publication Corporation.
[4] Angioni, A.; Barra, A.; Arlorio, M.; Coisson, J.D.; Russo, M.T.; Pirisi, F.F., et al. (2003). Chemical composition, plant genetic differences, and antifungal activity of the essential oil of Helichrysum italicum G. Don ssp. microphyllum (Willd) Nym. Journal of Agricultural and Food Chemistry, 51, 1030-1034.
[5] Apel, K.; Hirt, H. (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology, 55, 373–399.
[6] Ahmed M. (2012). Aboul-Enein, Faten Abu El-Ela, Emad A. Shalaby and Hany A. El-Shemy. Traditional medicinal plants research in Egypt: Studies of antioxidant and anticancer activities. Journal of Medicinal Plants Research, 6 (5), 689-703.
[7] Bakkali, F.; Averbeck, S.; Averbeck, D.; Idaomar, M. (2008). Biological effects of essential oils – A review. Food and Chemical Toxicology, 46, 446-475.
[8] Bora, K.S.; Sharma, A. (2011). The genus Artemisia: A comprehensive review. Pharmaceutical Biology, 49, 101–109.
[9] Brand-Williams, W., Cuvelier, M.E., Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28, 25–30.
[10] Cai, Y.; Luo, Q.; Sun, M.; Corke, H. (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Science, 74, 2157-2184.
[11] Daise Lopes-Luts, Daniela S.Alviano, Celuta S. Alviano, Paul P.Kolodziejczyk. (2008). Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry, 69, 1732-1738.
[12] El-Shemy H.A.; Aboul-Enein, A.M.; Aboul-Enein, K.M.; Fujita, K. (2007). Willow leaves’ extracts contain anti-Tumor agents effective against three cell types. PLOS ONE, 2(1), 178.
[13] Ferrigni, N.; Meyer, B.; Ferrigni, N.; Putnam, J.; Jacobsen, L.; Nichols, D.; Melaughlin, J. (1982). Brine shrimp: A convenient general bioassay for active plant constituents. Journal of Planta Medica, 45, 31-34.
[14] Grubov, V.I. (1982). Key to the Vascular Plants of Mongolia. Nauka: Leningrad, Russia. 245-253.
[15] Jian-Qing Yu, Jia-Chuan Lei, Xiu-Qiao Zhang, Huai-Dong Yu, Dai-Zhi Tian, Zhi-Xiong Liao, Guo-lin Zou. (2011). Anticancer, antioxidant and antimicrobial activities of the essential oil of Lycopus lucidus Turcz. var. hirtus Regel. Food chemistry,126,1593-1598.
[16] Kalemba, D., Kunicka, A. (2003). Antibacterial and antifungal properties of essential oils. Current Medicinal Chemistry, 10, 813–829.
[17] Markova, L.P. et al. (1985). Wild growing useful plants in flora Mongolia. Leningrad. 22, 89, 92.
[18] Lawrence, B.M., (2005). Antimicrobial/Biological activity of Essential Oils. Allured Publishing Corporation, Illinois, United States.
[19] Lee, J.; Godon, C.; Lagniel, G.; Spector, D.; Garin, J.; Labarre, J.; Toledano, M. B. (1999). Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast. Journal of Biological Chemistry, 274 (23), 16040.
[20] Ligaa, U., Davaasuren, B., Ninjil, N. (2005). Using the Mongolian medicinal herbs in eastern and western medicine. KSA Press, UB, Mongolia, 114.
[21] Martinez-Tome, M., Jimenez, A., Ruggieri, S., Frega, N., Strabbioli, R., et al. (2001). Antioxidant properties of Mediterranean spices compared with common food additives. Journal of Food Protection, 64, 1412-1419.
[22] Massada. Y. (1976). Analysis of Essential Oil by Gas Chromatography and Spectrometry. Wiley, New York, USA.
[23] Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26, 211-219.
[24] Moon, J.K.; Shibamoto, T. (2009). Antioxidant assays for plant and food components. Journal of Agricultural and Food Chemistry, 57(5), 1655-1666.
[25] Mosman, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. Journal of Immunological Methods, 65, 55-63.
[26] Sylvestre, M., Pichette, A., Longtin, A., Nagau, F., & Legault, J. (2006). Essential oil
[27] analysis and anticancer activity of leaf essential oil of Croton flavens L. From
[28] Guadeloupe. Journal of Ethnopharmacology, 103, 99-102.
[29] Salzer, U.J. (1977). The analysis of essential oils and extracts (oleoresins) from seasonings – A critical review. CRC Critical Reviews Food Science and Nutrition, 9, 345-373.
[30] Senatore, F., Arnold, N.A., Piozzi, F. (2004). Chemical composition of the essential oil of Salvia multicaulis Vahl. var. simplicifolia Boiss. growing wild in Lebanon. Journal of Chromatographia, 1052, 237-240.
[31] Shatar, S. (1989). Моngolian fragrant herbs. Soyombo Press, UB, Mongolia. 14-16.
[32] Shatar. S., Altantsetseg, Sh. (2000). Essential oil composition of some plants cultivated in Mongolian climate. Journal of Essential Oil Research, 12, 745-750.
[33] Sikkema, J., J.A. de Bont, Poolman, B. (1995). Mechanisms of membrane toxicity of hydrocarbons. Microbiological Reviews, 59, 201-222.
[34] Sylvester, P.W. (2011). Optimization of the tetrazolium dye (MTT) colorimetric assay for cellular growth and viability. Methods Molecular Biology, 716, 157-68.
[35] Tang, S. Y.; Whiteman, M.; Peng, Z. F.; Jenner, A.; Yong, E. L.; Halliwell, B. (2004). Characterization of antioxidant and antiglycation properties and isolation of active ingredients from traditional Chinese medicines. Free Radical Biology and Medicine, 36, 1575-1587.
[36] Valko, M.; Leibfritz, D.; Moncol, J.; Cronin, M.; Mazur, M.; Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. International Journal of Biochemistry and Cell Biology, 39, 44-84.
[37] Vandendool, H., Kratz, P.D. (1963). A generalization of the retention index system including linear temperature programmed gas liquid partition chromatography. Journal of Chromatographia, 11, 463–471.
[38] Verezovskaya, T.P. et al. (1991). Worm woods in Siberia. Novosibirsk. 48, 87-96.
[39] Zhang, X. (2004). “Traditional medicine: its importance and protection. In: Twarog S, Kapoor P (eds). Protecting and Promoting Traditional Knowledge: Systems, National Experiences and International Dimensions. Part 1. The Role of Traditional Knowledge in Healthcare and Agriculture”. New York: United Nations, 3-6.
How to Cite
Jambal, I., Ayurzana, A., & Shatar, A. (2023). ESSENTIAL OIL COMPOSITION AND ANTIOXIDANT, ANTICANCER ACTIVITIES OF ARTEMISIA FRIGIDA WILLD. GROWN IN MONGOLIA. IJRDO-Journal of Agriculture and Research (ISSN: 2455-7668), 9(5), 1-7.