Two Staining Methods in the Study of Ciliates
Keywords:
Ciliates, Paramecium, Carbol fuchsin, Acridine orange, Hoechst 33342
Abstract
Ciliates have two kinds of nuclei, somatic macrocnulei and germinal micronuclei. For the study of nuclear behaviour during their conjugation (sexual reproduction mode), nuclear staining techniques are very important. In the last decade, we studied nuclear behaviour of several species of Paramecium, and several nuclear staining methods were used. Here carbol fuchsin and ‘‘apofluor’’ (a double vital fluorescent dyes of acridine orange and Hoechst 33342) will be introduced.
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References
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[2] Gao X, Zhang X, Yang X. Morphological apoptotic characteristics of the post-meiotic micronuclei in Paramecium caudatum. Eur J Protistol, 2010, 46(3): 243-250.
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[13] Shi X, Frankel J. Morphology and development of mirror-image doublets of Stylonychia mytilus. J Protozool, 1991, 37(1): 1-13.
[14] Yang X, Shi X. Gametic nuclear exchange during the conjugation of Paramecium polycaryum. JPN J Protozool, 2007, 40: 113-121.
[15] Ishida M, Nakajima Y, Kurokawa K, Mikami K. Nuclear behavior and differentiation in Paramecium caudatum analyzed by immunofluorescence with anti-tubulin antibody. Zoo Sci, 1999, 16: 915-926.
[16] Yang X, Takahashi M. Nuclei may anchor at specific locations during nuclear determination in Paramecium caudatum. Eur J Protistol, 2002, 38(2): 147-153.
[17] Carr DH, Walker JE. Carbol fuchsin as a stain for human chromosome. Biotech Histochem, 1961, 36(4): 233-236.
[18] Yang X, Takahashi M. Stage-related profiles of the reaction of the cells to heat shock during meiotic prophase in Parameicum caudatum. J Exp Zool, 1996, 275: 53-60.
[19] Santos ML, Lu E, Wolfe J. Nuclear death in living Tetrahymena: the case of the haploid nuclei. J Eukaryot Microbiol, 2000, 47: 493-498.
[20] Yang X, Gao X, Shi X. Detection of haploid nuclear death in living Paramecium caudatum. JPN J Protozool. 2007, 40: 123-130.
[21] Yang X, Gao X. Introduction of an efficient method for the observation of living Paramecium. IJRDO-Journal of Education Research, 2018, 3(10): 27-29.
[22] Gao X, Yang X. Introduction of an effective method for separating conjugating pairs of Paramecium. IJRDO-Journal of Biological Science, 2019, 5(10): 1-3.
[23] Gao X, Yang X. Increasing the yield of iron-dextran nanoparticles by ultrasonic treatment. IJRDO-Journal of Biological Science, 2019, 5(10): 4-6.
[24] Yang X, Zhang X, Huang X. The application of vital fluorescence dyes in the study of ciliates. Acta Hydrobiol Sinica, 2008, 32(suppl): 80-83. (in Chinese with English abstract)
[2] Gao X, Zhang X, Yang X. Morphological apoptotic characteristics of the post-meiotic micronuclei in Paramecium caudatum. Eur J Protistol, 2010, 46(3): 243-250.
[3] Gao X, Shi X, Yang X. Dynamic behaviour of stationary pronuclei during their positioning in Paramecium caudatum. Eur J Protistol, 2011, 47(3): 235-237.
[4] Gao X, Xu C, Yang X. Characterization of meiotic nuclear degeneration in Paramecium tetraurelia. Chn J Zoo, 2011, 46(3): 89-92. (in Chinese with English abstract)
[5] Gao X, Zhu J, Yang X, Yuan J, Wang Y, Song M. Localization of stationary pronuclei during the conjugation of Paramecium indicated by immunofluorescence staining. Zool Res, 2011, 32(4): 461-464.
[6] Gao X, Yang X, Zhu J, Yuan J, Wang Y, Song M. New details indicated by different stainings during the conjugation of ciliated protozoa Paramecium. Zool Res, 2011, 32(6): 651-656.
[7] Wang Y, Yuan J, Gao X, Yang X. Stage-specific appearance of cytoplasmic microtubules around the surviving nuclei during the third prezygotic division of Paramecium. Zool Res, 2012, 33(E5-6): E97-E102.
[8] Gao X, Song Y, Yang X. Nuclear behavior in exconjugants of Paramecium bursaria. IJRDO-Journal Biological Science, 2018, 4(7): 1-5.
[9] Yang X, Takahashi M. Disturbance of the determination of germinal and somatic nuclei by heat shock in Paramecium caudatum. J Eukaryot Microbiol, 1999, 46(1): 49-55.
[10] Lin Y, Gao X, Yang X. A method for observation of living Paramecium. Shichuan J Zool, 2009, 28: 276. (in Chinese with English abstract)
[11] Vosskühler C, Tiedtke A. Magnetic separation of phagosomes of defined age from Tetrahymena thermophila. J Eukaryot Microbiol, 1993, 40: 556-562.
[12] Sun X, Wu J, Gao X and Yang X. To enhance the production of magnetic iron-dextran particles by ultrasonic treatment and its applications in ciliate studies. Chn J Zoo, 2010, 45(1): 90-93. (in Chinese with English abstract)
[13] Shi X, Frankel J. Morphology and development of mirror-image doublets of Stylonychia mytilus. J Protozool, 1991, 37(1): 1-13.
[14] Yang X, Shi X. Gametic nuclear exchange during the conjugation of Paramecium polycaryum. JPN J Protozool, 2007, 40: 113-121.
[15] Ishida M, Nakajima Y, Kurokawa K, Mikami K. Nuclear behavior and differentiation in Paramecium caudatum analyzed by immunofluorescence with anti-tubulin antibody. Zoo Sci, 1999, 16: 915-926.
[16] Yang X, Takahashi M. Nuclei may anchor at specific locations during nuclear determination in Paramecium caudatum. Eur J Protistol, 2002, 38(2): 147-153.
[17] Carr DH, Walker JE. Carbol fuchsin as a stain for human chromosome. Biotech Histochem, 1961, 36(4): 233-236.
[18] Yang X, Takahashi M. Stage-related profiles of the reaction of the cells to heat shock during meiotic prophase in Parameicum caudatum. J Exp Zool, 1996, 275: 53-60.
[19] Santos ML, Lu E, Wolfe J. Nuclear death in living Tetrahymena: the case of the haploid nuclei. J Eukaryot Microbiol, 2000, 47: 493-498.
[20] Yang X, Gao X, Shi X. Detection of haploid nuclear death in living Paramecium caudatum. JPN J Protozool. 2007, 40: 123-130.
[21] Yang X, Gao X. Introduction of an efficient method for the observation of living Paramecium. IJRDO-Journal of Education Research, 2018, 3(10): 27-29.
[22] Gao X, Yang X. Introduction of an effective method for separating conjugating pairs of Paramecium. IJRDO-Journal of Biological Science, 2019, 5(10): 1-3.
[23] Gao X, Yang X. Increasing the yield of iron-dextran nanoparticles by ultrasonic treatment. IJRDO-Journal of Biological Science, 2019, 5(10): 4-6.
[24] Yang X, Zhang X, Huang X. The application of vital fluorescence dyes in the study of ciliates. Acta Hydrobiol Sinica, 2008, 32(suppl): 80-83. (in Chinese with English abstract)
Published
2019-11-30
How to Cite
Yang, X., & Xin Gao. (2019). Two Staining Methods in the Study of Ciliates. IJRDO- Journal of Educational Research, 4(11), 33-35. https://doi.org/10.53555/er.v4i11.3362
Section
Articles
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