EFFECT OF WET MASH WITH OR WITHOUT ADDED PHYTASE ON PHYTATE-P AVAILABILITY: IN VITRO HYDROLYSIS OF PHYTATE

  • Salah Khatir Jubarah University of Juba
  • Milton L. Melingasuk University of Juba
  • Rizig E. Loma Marial Luo Livestock Training Centre, Tonj State, South Suda
Keywords: Phytate-P, Phytase, Dietary-wetness, Inorganic phosphorus, in vitro

Abstract

Dietary wetness is envisaged to augment phytate hydrolysis through enhanced phytate accessibility to phytase, since phytate degradation is water-mediated. In determining the influence of dietary wetness with supplementary phytase on phytate-P availability, four plant-based growers diets were formulated, containing 0.0, 0.1, 0.2 and 0.3g kg-1 added phytase and with the required phosphorus being entirely phytate-P. Dietary moisture contents were reconstituted by addition of three levels of 0.0, 30 and 60 % distilled-deionised water on Dry Matter basis (DM). Dietary treatments were arranged in a factorial randomized complete block design with three replications. The released inorganic phosphorus was determined at 6hr intervals throughout the 24hr incubation period at 24ºC. The 30% and 60% dietary wetness with added phytases significantly increased (P<0.05) inorganic phosphorus output compared to either 0.0% or wetness without added phytase.  At 6hr of incubation, phytase plus 60% wetness had the highest (P<0.05) inorganic phosphorus, while at 12hr 30% dietary wetness plus phytase yielded the highest inorganic phosphorus (P<0.05). Wetness plus phytase had no pronounced effect (P<0.05) beyond 12hr, except at 24hr (P<0.05). Increasing supplementary phytase in association with dietary wetness significantly (P<0.05) increased inorganic phosphorus output, however, setting wet diets beyond 12hr, produced gases and dietary discoloration.

Author Biographies

Salah Khatir Jubarah, University of Juba

Associate Professors,  Department of Animal Production, College of Natural Resources and Environmental Studies, University of Juba, P.O. Box 82 Juba, South Sudan

Milton L. Melingasuk, University of Juba

Associate Professors,  Department of Animal Production, College of Natural Resources and Environmental Studies, University of Juba, P.O. Box 82 Juba, South Sudan

Rizig E. Loma, Marial Luo Livestock Training Centre, Tonj State, South Suda

Technical Officer, Marial Luo Livestock Training Centre, Tonj State, South Suda

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Published
2018-01-31